*Applications will be reviewed on a rolling-basis.
A research opportunity is currently available with the National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP) at the Centers for Disease Control and Prevention (CDC) located in Atlanta, Georgia.
The purpose of the project “Macaque models of HIV treatment and prevention” is to identify in animal models novel long-acting HIV treatment and prevention modalities that can i) maximize virus suppression in persons living with HIV or ii) prevent infection when used for pre-exposure prophylaxis (PrEP) in uninfected individuals. The selected participant will be paired with a mentor within the HIV Laboratory branch at the Division of HIV/AIDS Prevention at CDC. The Laboratory Branch has unique expertise in drug pharmacokinetics and macaque models of virus suppression and PrEP. The research assignment requires the use of molecular assays to detect and characterize RNA and DNA sequences in animals infected with simian HIV (SHIV). Specific activities will include:
- Perform a variety of molecular and serologic diagnostic assays to monitor infection with SHIV in macaques.
- Prepare antiretroviral drug cocktails and SHIV stocks for in vivo studies in macaques.
- Process blood, mucosal fluids, and tissues using established procedures and make adjustments as needed. Assist in the isolation of cell subpopulations from tissues for antiretroviral drug measurements.
- Perform molecular biology techniques including cloning and sequence analysis to characterize viruses from infected animals, and assess antiretroviral drug resistance profiles and evolution of viral genomes.
- Propagate and characterize new virus stocks in vitro including their infectious titer and phenotypic susceptibility to antiretroviral drugs.
- Develop and apply new PCR assays and make adjustments to improve assay performance.
If you have questions, send an email to ORISE.CDC.NCHHSTP@orau.org. Please include the reference code for this opportunity (CDC-NCHHSTP-2021-0061) in your email.